An ALEXYS method has been developed for the analysis of Azithromycin following the official USP method. The USP method for Azi uses two flow cells in series, cell 1 for screening and cell 2 for detection of the analytes. The first flow cell is a Reactor cell, the second is the SenCell. Under the specified LC conditions all USP criteria are met. Chromatogram: a mixture of several macrolides was analysed to illustrate the chromatographic performance of the method. All components are separated with at least a resolution of 2.5.
- Excellent reproducibility and linearity
- In compliance with USP requirements
- Total analysis time <14 min
Configuración del sistema:
For the analysis of Azythromycin we selected the SenCell. This cell not only has an excellent reproducibility but also the best possible detection sensitivity. A Reactor was used as pre-oxidation screening cell to meet USP requirements. The ALG is a USP L49 type of column.
The ALEXYS system comprises the following parts:
- AS 110 autosampler, cool
- LC 110S HPLC pump
- OR 110 organiser rack
- Clarity data acquisition software
- DECADE II electrochemical detector, DCC
- SenCell with GC WE, sb REF
- Flexcell with GC WE and HyREF
