CDT in Serum
CDT (Carbohydrate Deficient Transferrin) is the most specific parameter for chronic alcohol abuse.
Manual Sample preparation.
The ClinRep® HPLC Complete Kit for CDT provides a unique chromatographical resolution compared to other commercially available test kits. The analytical method correlates with the reference method of the IFCC-CDT-WG* and ensures a reliable separation of all relevant transferrin isoforms (a-, mono-, di-, …, hexasialotransferrin). This applies in particular for disialotransferrin, which is the defined analyte by this group. Alternatively to this method with manual sample preparation, ClinRep® Complete Kits for on-line analysis and automated on-line analysis via 96-well plates are available.
*Working group of the IFCC for the international standardisation of CDT measurement.
On-line Analysis.
This ClinRep® Complete Kit is designed for the fast analysis of CDT with on-line SPE HPLC. It features an injection interval of 9.5 min only and provides a unique chromatographical resolution compared to other commercially available test kits. Analogously to our kit with manual sample preparation, the on-line procedure correlates with the candidate reference method of the IFCC-CDT-WG*. Prior to the sample injection, a simple dilution step is required (”sample pre-treatment“, see below). Alternatively, the dilution can be performed automated via 96-well plates. For this purpose an additional ClinRep® Complete Kit is available.
Automated on-line Analysis.
This ClinRep® Complete Kit is designed for the fully automated analysis of CDT with liquid handling systems and subsequent on-line SPE HPLC. The analytical method correlates with the candidate reference method of the IFCC-CDT-WG* and ensures a reliable separation of all relevant transferrin isoforms (a-, mono-, di-, …, hexa-sialotransferrin). This applies in particular for disialotransferrin, which is the defined analyte by this group. An accurate calculation of the CDT value is ensured by baseline separation.
* Working group of the IFCC for the international standardisation of CDT measurement.
We have available an user manual for this application. You can request sending an email to clinica@ingenieria-analitica.com
| Order No. | Description | Quantity |
| 21100 | ClinRep® Complete Kit for CDT | 1 pce. |
| (Carbohydrate-deficient Transferrin) | ||
| in Serum | ||
| for 300 assays | ||
| Contents: | ||
| Mobile Phase A | 3 x 21007 | |
| Mobile Phase B | 1 x 21008 | |
| Mobile Phase C | 2 x 21009 | |
| Vials for Sample Pretreatment with Diluting Solution | 6 x 21120 | |
| Manual | ||
| Quick Reference | ||
| Separately available components: | ||
| 21007 | Mobile Phase A | 2000 ml |
| 21008 | Mobile Phase B | 2000 ml |
| 21009 | Mobile Phase C | 2000 ml |
| 21013 | Serum Calibrator Set, lyophil. (Level 1 – 2) | 2 x 1 x 1 ml |
| 21014 | Test Solution | 15 ml |
| 21120 | Vials for Sample Pretreatment with Diluting Solution | 50 pcs. |
| Start Accessories: | ||
| 21030 | Analytical Column with test chromatogram | 1 pce. |
| 21032 | Cartridge incl. 1 Precolumn | 1 pce. |
| FK5820 | Stainless steel prefilter holder incl. 1 prefilter | 1 pce. |
| FK7400 | Inline-Filter (stainless steel sieve, free of dead volume) | 1 pce. |
| Refill Service: | ||
| 21031 | Analytical Column with test chromatogram | 1 pce. |
| (original column needs to be returned) | ||
| Accessories: | ||
| 21033 | Precolumns | 5 pcs. |
| FK1102 | Switching valve Sykam (6-port / 3-channel incl. electr. drive, PEEK) | 1 pce. |
| FK5810 | PEEK tubing (connection of prefilter and column) | 1 pce. |
| FK5821 | Replacement filter for Stainless steel prefilter holder | 10 pcs. |
| FK7340 | Sealings and sieves for order no. FK7400 | 4 pcs. each |
| FW3000 | Fixed-Wavelength-VIS-Detector (460 nm), complete | 1 pce. |
| RC2002 | Sodium-Hypochlorite-Solution (5 %) | 12 ml |
| ClinChek® Controls: | ||
| 21080 | Serum Control, lyophil. | 10 x 1 ml |
| Level I | ||
| 21081 | Serum Control, lyophil. | 10 x 1 ml |
| Level II | ||
| 21082 | Serum Control, lyophil. | 2 x 5 x 1 ml |
| Level I, II |
