Autosampler 3950 HPLC
The Autosampler 3950 can inject from up to 768 positions when equipped with microtiter plates (either high or low formats) or from up to 96 standard 2 ml sample vials. Sample carryover is significantly minimized thanks to a highly-effective interior and exterior needle wash procedure. This autosampler is also fast and flexible: one complete sample injection cycle takes less than one minute, including needle wash. Three different injection modes are supported; “full loop filling” (highest precision and reproducibility), “partial loop filling” (variable volumes, e.g. for dilution series) and “µl pickup” (loss-free injection of extremely small sample volumes), allowing the user to optimize sample usage. The headspace pressure function prevents bubbles from forming in the vial during sample uptake. Precolumn derivatization is supported.
For high-pressure injections of up to 1000 bar, the autosampler is equipped with a so-called ILD™ valve (Intermediate Loop Decompression). This valve consists of a rotor-stator combination and includes a central port for depressurizing. For high pressure applications, the sample loop is depressurized prior to receiving the sample. This way, the sample is not diluted with solvent. Because the valve is switched extremely fast, pressure spikes are reduced. Analyses are more precise and wear of the column is reduced.
Key features:
- 1000 bar
- 0.1-5000 µl injection volume
- PASATM (pressure-assisted sample aspiration)
- ILD (intermediate loop decompression)
- Cooling/heating version (4-40°C)
- With sample tray cooling (4 - 22°C) and biocompatible sample path.
Different types of HPLC Autosamplers 3950:
- Standard version of a fast and very versatile analytical HPLC autosampler, 1000 bar, 0.1- 5000 µl injection volume.
- Cooling/heating version of a fast and very versatile analytical HPLC autosampler, 1000 bar, 0.1-5000 µl injection volume.
- Analytical HPLC autosampler with biocompatible flow path.
- Analytical HPLC autosampler with biocompatible flow path and sample cooling
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Sample injection |
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Sample capacity |
microtiter plates for max. 768 samples or sample plates for max. 96 vials |
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Vial/plate dimensions |
Max. plate/vial height: 47 mm (incl. septa or capmat) |
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Injection volume range |
0.1–5000 µl programmable |
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Sample loop |
10 µl standard |
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Dispenser syringe |
250 µl |
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Headspace pressure |
built-in compressor, only for vials with septum |
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Switching time inj. valve |
< 100 ms |
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Piercing needle precision |
± 0.6 mm |
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Sample tray cooling |
None (STANDARD VERSION) 4-40 °C (COOLING VERSION) |
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Vial detection |
missing vial/well plate detection by sensor |
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Needle wash |
programmable: wash between injections and wash between vials |
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Wetted materials |
Stainless steel (SS316), Tefzel®, Vespel®, glass, Teflon® (PTFE). |
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Analytical performance |
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Injection modes |
full loop, partial loopfill and µl pickup mode; PASA™ (pressure-assisted sample aspiration) |
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Injection precision |
RSD (Relative Standard Deviation): full loop injection: <0.3% partial loop injection at an injection volume >10 µl: <0.5% microliter pickup injection at an injection volume >10 µl: <1.0% |
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Injection volumes |
full loop: max. 5000 µl 1 µl increment for all injection modes |
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Sample carryover |
< 0.05 % with programmable needle wash |
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Injections per vial |
max. 9 injections |
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Injection cycle time |
minimum 7 s from the same vial, 14 s from different vials; < 60 s for ≤ 100 µl sample injection in all injection modes, incl. 300 µl needle wash |
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Analysis time |
max. 9 h, 59 min, 59 s |
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Communication |
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Inputs |
2 programmable TTL inputs (next injection, freeze, stop) |
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Outputs |
1 programmable relay output (inject marker, auxiliary, alarm) |
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Control |
Ethernet (LAN) |
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Technical parameters |
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Ambient conditions |
temperature range: 4–40 °C; 39.2–104 °F air humidity: below 90 % humidity (non condensing) |
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General |
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Power supply |
90–230 V AC ± 10 %; 50–60 Hz; 200 VA |
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Dimensions |
300 x 360 x 510 mm (W x H x D) |
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Weight |
19.0 kg |
