ALEXYS 3-Nitrotyrosine Analyzer
Peroxynitrite has been reported to react with L-tyrosine to produce 3-nitro-L-tyrosine (NO2-Tyr), which appears to be a suitable marker for ONOO- mediated tissue damage. The NO2-Tyr standards are analyzed in a dual cell configuration with a detection limit of 0.5 nmol/L. As conversion in the first flow cell increases with lowering the flow rate, a micro LC (1mm) system is required. The ALEXYS system is optimised for analysis of small sample volumes and best detection performance.
- Dual flow cell setup, for better selectivity
- Microbore HPLC, for better sensitivity
- Small sample volume
- Detection limit 0.5 nmol/L
For the analysis of nitrotyrosine we selected the Sencell. This cell not only has an excellent reproducibility but also the best possible detection sensitivity. We routinely inject 2-5 µL with a maximum sample use of only 10 µL. Therefore, we selected a micro version of our AS 110 autosampler together with a micro LC 110 pump. We use a 1 mm ID column at 50 µL/min as a standard.
The ALEXYS system comprises the following parts:
- AS 110 autosampler, cool, micro
- LC 110S HPLC pump
- OR 110 organiser rack
- Clarity data acquisition software
- DECADE II DCC electrochemical detector
- SenCell with GC WE, salt bridge
- Reactor cell with GC WE, HyREF
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Part Number |
Description |
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180.0072C |
ALEXYS Nitrotyrosine Analyzer |
