1260 Infinity Binary LC PAD System

The 1260 Infinity Binary LC system with PAD (Pulsed Amperometric Detector) yields faster results with significantly higher data quality. Delivering a pressure of 600 bars, this system boasts flat baselines and impressive sensitivity with the Electrochemical  Detector (EC), thanks to the superior gradient accuracy from high pressure mixing. Based on the highly successful 1200 Series Rapid Resolution LC System, we promise full backwards compatibility for your established HPLC and RRLC methods. The binary system supports a wide range of LC and LC/MS applications with any narrow and standard bore analytical column.

The addition of the Electrochemical Detector as an integrated Channel Partner complete solution opens the HPLC technique to unexplored sensitivity limits for specific applications where the EC technology is required.

APPLICATION AREAS

Neuroscience

  •  Monoamines and Metabolites
  •  GABA and Glutamate
  •  Choline and Acetylcholine
  •  Online MD of Monoamines
  •  Glutathione and disulfides
  •  Nitrotyrosine

 Pharmaceutical & Biotech

  •  Aminoglycoside antibiotics
  •  Antipsychotic drugs
  •  Azithromycin
  •  Betadex Sulfobutyl Ether Sodium
  •  Epinephrine in anaesthesia
  •  Fluorodeoxyglucose
  •  Glutathione and disulfides
  •  Heparin

Clinical & Diagnostics

  • Catecholamines in Urine or Plasma
  • Serotonin in Urine or Plasma
  • Metanephrines in Urine
  • VMA HVA 5 HIAA in Urine
  • Homocysteine in Plasma
  • Fluorodeoxyglucose
  • Vitamins
  • Iodide in Urine

Food and Environment

  • EPA Phenols in water and soil
  • Bisphenol A in water
  • Polyphenols in food
  • Carbohydrates in food
  • Lactose-free products
  • Iodide in milk
Specifications Agilent 1260 Infinity Binary Pump (G1312B) and VL version (G1312C)
Hydraulic system Two dual piston in series pumps with servo-controlled variable stroke drive, floating pistons
Settable flow range Set points 0.001 - 5 mL/min, in 0.001 mL/min increments
Flow range 0.05 - 5.0 mL/min
Flow precision ≤ 0.07% RSD or ≤ 0.02 min SD, whatever is greater; based on retention time at constant room temperature
Flow accuracy ± 1 % or 10 µL/min, whatever is greater; pumping degassed H2O at 10 MPa
(100 bar)
Pressure operating range 1260 Infinity Binary Pump:
Operating range up to 60 MPa (600 bar, 8700 psi) up to 5 mL/min

1260 Infinity Binary Pump VL:
Operating range up to 40 MPa (400 bar, 5880 psi) up to 5 mL/min
Pressure pulsation 1260 Infinity Binary Pump:
< 2 % amplitude (typically < 1.3 %) or < 0.3 MPa (3 bar), whatever is greater, at 1 mL/min isopropanol, at all pressures > 1 MPa (10 bar, 147 psi) Low delay volume configuration: < 5% amplitude (typically < 2 %) 

1260 Infinity Binary Pump VL:
< 2 % amplitude (typically < 1.3 %) or < 0.3 MPa (3 bar), whatever is greater, at 1 mL/min isopropanol, at all pressures > 1 MPa (10 bar, 147 psi)
Compressibility compensation 1260 Infinity Binary Pump:
Pre-defined, based on mobile phase compressibility

1260 Infinity Binary Pump VL:
User selectable, based on mobile phase compressibility
Recommended pH range 1.0 - 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel
Gradient formation High-pressure binary mixing
Delay volume 1260 Infinity Binary Pump: 
Standard delay volume configuration: 600 - 800 μL (includes 400 μL mixer), dependent on back pressure; measured with water at 1 mL/min (water/caffeine tracer) 
Low delay volume configuration: 120 µL

1260 Infinity Binary Pump VL:
600 - 900 µL (includes 400 μL mixer), dependent on back pressure
Composition range Settable range: 0 - 100 %
Recommended range: 1 - 99 % or 5 µL/min per channel, whatever is greater
Composition precision < 0.15 % RSD or < 0.04 min SD, whatever is greater, at 0.2 and 1 mL/min; based on retention time at constant room temperature
Composition accuracy 1260 Infinity Binary Pump:
± 0.35 % absolute, at 2 mL/min, at 10MPa (100 bar), (water/caffeine tracer)

1260 Infinity Binary Pump VL:
< 0.5 % absolute
Control Agilent control software (e.g. ChemStation, EZChrom, OL, MassHunter)
Local control Agilent Instant Pilot
Communications Controller-area network (CAN), RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN optional
Safety and maintenance Extensive diagnostics, error detection and display through Agilent LabAdvisor, leak detection, safe leak handling, leak output signal for shutdown of the pumping system. Low voltage in major maintenance areas.
GLP features Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of seal wear and volume of pumped mobile phase with pre-defined and user settable limits and feedback messages. Electronic records of maintenance and errors
Housing All materials are recyclable

 

 Specifications DECADE ELITE

 Power

 100-240 VAC, 50/60 Hz, 260 VA max., auto-sensing

 Operation  modes

 DC, Pulse and Scan mode

 Potential range

 ± 2.50 V, 10 mV increments

 Output (DAC)

 ± 1 V (16-bit D/A converter)

 Output (I/E)

 ± 2.5 V (unprocessed analog signal)

 Auto zero

 triggered by keyboard, rear panel TTL, or control software

 LAN

 instrument control, data acquisition (USB service port)

 Oven

 from +7°C above ambient to 60°C, accuracy 0.5°C, stability 0.1°C; accommodates column and flow cell(s)

 Regulatory

 CE, cMETus approved, RoHS compliant

 Rear panel I/O

 1x IEC inlet (Mains), 1x USB B, 1x RJ45 LAN, 1x 9-pins sub-d D Male (Valve), 1x 9-pins sub-d Female (Analog output), 1x 25-pins sub-d  Female (Digital I/O)

 Filter

 Advanced digital filter (10 - 0.001 Hz in 1, 2, 5 increments)

 DC mode

 Range

 10 pA - 200 µA in 1, 2, 5 increments

 Data Rate

 1 - 100 Hz in 1, 2, 5 increments (actual data rate dependent on filter setting)

 Noise

 ≤ 2 pA with dummy cell (load of 300 MΩ/0.5 µF), range 1 nA/V, filter off, Ec +800mV and temperature of 35 °C.

 Pulse mode

 Range

 10 nA - 200 µA in 1, 2, 5 increments

 Waveform

 5-step potential pulse (max)

 Data Rate

 1/(pulse duration) Hz

 Pulse times

 t1: 100 ms - 2000 ms; t2, t3, t4, t5: 0 - 2000 ms in 10 ms increments

 Sampling times

 ts: 20 ms to (t1 - 60 ms)

 Scan rate

 Range

 10 nA - 200 µA in 1, 2, 5 increments

 Data Rate

 1 Hz

 Scan rate

 1 - 100 mV/s in 1, 2, 5 increments, cycle: half, full or continuous

 Physical specifications

 Dimensions

 43 (D) x 22 (W) x 44 (H) cm = 16.9” (D) x 8.7” (W) x 17.3” (H)

 Weight

 max 14.4 kg (32 lbs) without flow cell and column