ALEXYS Aminoglycoside Analyzers
The Aminoglycoside Analyzers are a number of system solutions for the analysis of Neomycin, Framycetin, Tobramycin, Lincomycin, Gentamicin, Spectinomycin, Kanamycin and Amikacin. The system is dedicated to the analysis of these antibiotics in pharmaceutical formulations and bulk product. Aminoglycosides are manufactured by a fermentation process and the main constituents as well as impurities and by-products must be identified and quantified. The system uses reversed phase chromatography with post column addition of sodium hydroxide for pulsed amperometric detection. Except for the Kanamycin/Amikacin analyzer which uses an ion exchange column.
Above chromatogram is an example of Aminoglycoside analysis. It shows a repeatability study, an overlay of Spectinomycin (left, n=6) and Gentamycin (right, n=7) chromatograms.
* In a few cases the USP/EP conditions were modified, to improve assay specifications regarding reproducibility and peak resolution
For the analysis of aminoglycosides we selected the Flexcell electrochemical cell. This cell has an exchangeable working electrode which is particularly useful in pulsed electrochemical detection. The electrode material tends to vanish over the years, in the process of pulsed amperometric detection. Exchanging an electrode disc only takes a few minutes. The HyREF is completely maintenance free even at pH 13 which is required for pulsed amperometric detection! The ALEXYS system comprises the following parts:
- AS 110 autosampler, cool
- LC 110S HPLC pump (2)
- OR 110 organiser
- Clarity data acquisition software
- DECADE II electrochemical detector
- Flexcell with Au WE, HyREF
Aminoglycosides 180.0050/56/59 require post-column addition of sodium hydroxide for detection. System 180.0058C is based on ion exchange chromatography at high pH (no post column addition). In addition to the first mentioned list this system includes Helium-proof solvent bottles.
|
Part no |
Description |
|
180.0050C |
ALEXYS Aminoglycosides Analyzer |
|
180.0056C |
ALEXYS Gentamicin Analyzer |
|
180.0058C |
ALEXYS Kanamycin/Amikacin Analyzer |
|
180.0059C |
ALEXYS Spectinomycin/Lincomycin Analyzer |
