Products

1260 Infinity Fluorescence Detector Spectra

Lower detection limits and easy spectral analysis for HPLC and UHPLC 

As a standalone detector or in an automated Agilent 1200 Infinity Series system the Agilent 1260 Infinity Fluorescence Detector Spectra brings high-sensitivity fluorescence detection to your laboratory. This easy-to-use detector provides quantitative data and fluorescence spectra from a single run. Simultaneous multi-wavelength detection improves sensitivity and selectivity. Use the online spectral information for rapid method optimization and verification of separation quality. High-speed fluorescence detection with up to 145 Hz data rates keeping pace with the analysis speed of ultra-fast LC.

  • Rotating gratings for multi-signal and online spectral data acquisition without loss in sensitivity.

  • Lowest limits of detection with a Raman S/N > 3000 (using dark signal noise reference).

  • Spectra and quantitative data from a single run.

  • View online spectra without interrupting the chromatographic run.

  • Simplified optical design for optimized baseline stability.

  • Up to 100% resolution gain in fast LC using a 145 Hz data acquisition rate.

  • Long-life xenon lamp for highest sensitivity. The long-life (> 4000 hours) flash lamp, lamp reference system and efficient light collection ensure constant lamp energy for maximum excitation of fluorophores.

  • Easy front access enables fast inspection or exchange of the flow cell.

  • Automatic recognition of all flow cell cartridges provides documentation of instrument parameters and helps to comply with GLP.

  • Extensive diagnostics, error detection and display with Instan Pilot controller and Agilent Lab Advisor Software

Fluorescence Detector 1260 Infinity Spectra (G1321B)

Specifications: Agilent 1260 Infinity Fluorescence Detector  Spectra (G1321B)

Detection type

Multi-signal fluorescence detector with rapid online scanning capabilities and spectral data analysis

Performance

Single wavelength operation:
a) Raman (H2O) > 500 (noise reference measured at signal)
Ex 350 nm, Em 397 nm, dark value 450 nm, standard flow cell
b) Raman (H2O) > 3000 (noise reference measured at dark value)
Ex 350 nm, Em 397 nm, dark value 450 nm, standard flow cell Dual wavelength operation:
Raman (H2O) > 300 Ex 350 nm, Em 397 nm and Ex 350 nm, Em 450 nm, standard flow cell

Light source

Xenon flash lamp, normal mode (20 W), economy mode (5 W), lifetime 4000 hours

Pulse frequency

296 Hz for signal mode, 74 Hz for economy mode

Excitation monochromator

Concave holographic grating, F/1.6, blaze 300 nm,
Range 200 – 1200 nm and zero-order, bandwidth 20 nm

Emission monochromator

Concave holographic grating, F/1.6, blaze 400 nm,
Range: settable 200 – 1200 nm and zero-order, bandwidth 20 nm

Reference system

Inline excitation measurement

Time programming

Up to four signals, response time, PMT gain, baseline behavior (append, free, zero), spectral parameters

Spectral acquisition

Excitation or emission spectra, scan speed 28 ms per datapoint, e.g. 0.6 s per spectrum 200-400 nm, 10 nm step

Step size

1-20 nm

Spectra storage

Limited only by disk space

Wavelength repeatability

± 0.2 nm

Wavelength accuracy

±3 nm

Data rate

145 Hz

Flow cells

Standard: 8 µL volume, 20 bar (2 MPa) pressure maximum, quartz.
Optional: 
Fluorescence cuvette for offline spectroscopic measurements with 1 mL syringe, 8 µL volume, quartz
Semi-micro flow cell, 4 µL, 20 bar pressure maximum 
Bio-inert flow cell, 8 µL, 20 bar pressure maximum

Control and data evaluation

Agilent OpenLAB Chromatography Data System (CDS) ChemStation Edition and EZChrom Edition  or Agilent Instant Pilot data evaluation (limited analysis or printing of spectra)

Environment

0 - 40 °C constant temperature at <95% humidity (non-condensing)

Analog outputs

Recorder/integrator: 100 mV or 1 V,
Output range selectable from 0.2 to 400 LU, 2 outputs

Communications

LAN, Controller-area-network (CAN), RS232, APG remote
Remote: ready, start, stop and shut-down-signals

Safety and maintenance

Extensive diagnostics, error detection and display (through ChemStation and Instant Pilot), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas

GLP

Early maintenance feedback (EMF) for continuous tracking of instrument usage, display of feedback messages in terms of lamp burn time with user-settable limits and feedback messages. Electronic records of maintenance and errors. Verification of wavelength accuracy using Raman band (H20) lines.

 

 

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