Products

325 Dual Wavelength UV-Vis Detector

The Agilent 325 Dual Wavelength UV-Vis Detector delivers superb sensitivity for detection  in the UV and Visible light ranges. Dual path flow cell technology offers a dynamic absorbance range 40 times greater than conventional detectors, especially for preparative LC applications.

  • Secure compound identification - simultaneously handling two user-defined UV or visible wavelengths increases analyte information and enhances productivity. Peak purity is easily assessed with dual wavelength and peak ratio capabilities.

  • Superb dynamic range – Dual Pathlength flow cell delivers a dynamic absorbance range 40 times greater than conventional detectors.

  • Scale-up easily from analytical to preparative flow rates. Interchangeable, cartridge-style flow cells quickly switch flow rates from a microliter per minute to hundreds of milliliters per minute.

  • OpenLAB CDS ChemStation Edition Software or LC Responder Software provide full system control, data analysis and multiple reporting options plus tools for 21 CFR Part 11 compliance.

  • Stable, precise wavelength settings – Deuterium source is used for the ultraviolet range and a quartz halogen lamp for the visible range.

  • Tool-free maintenance – Routine maintenance is easy with front panel access. Simple thumbscrews secure the lamps and flow cells, no tools are needed.

  • Regulatory compliance – Agilent offers complete IQ, OQ, and PQ documentation packages and contract services to help meet regulatory requirements.

Especificaciones Agilent 325 con doble longitud de onda UV-Vis

Wavelength range

190 to 900 nm

Light sources:

Deuterium for UV and quartz halogen for Vis

Spectral bandwidth:

6 nm

Wavelength precision:

±0.1 nm

Wavelength accuracy:

±1 nm at 260 nm

Noise, single wavelength:

±5 x 10-6 AU peak-to-peak at 254 nm, 2 sec response time, 1 mL/min MeOH, 9-mm flow cell

Noise, dual wavelength:

±10 x 10-6 AU peak-to-peak at 254/280 nm, 
2 sec response time, 1 mL/min MeOH, 9-mm flow cell

Noise, dry cell:

Typically, ±3 x 10-6 AU peak-to-peak at 254 nm, 
2 sec response time, 9 mm flow cell

Drift:

Less than 1 mAU/hr

Linearity, with acetone in water:

Analytical 1% to 2 AU; preparative1% to 20 AU at 265 nm

Absorbance range:

Up to 70 AU with preparative flow cell

Response time, selectable:

0.05, 0.5, 1, and 2 sec

Data rate:

20 Hz single channel

Analog outputs:

Two with selectable absorbance range of 0.001 to 100 AU full scale at 1V

External:

Ethernet

Contact closure outputs:

Four time-programmable external event relays, one peak sense relay, three synchronization signals (ready out,start out, and fault out)

Contact closure inputs:

Lamp off, autozero, three synchronization signals (ready in, start in, and fault in)

Software:

Agilent OpenLab ChemStation 

Dual channel ratio calculation:

Peak detection through OpenLab ChemStation

Dual pathlength flow cells:

Preparative flow cell:  9 mm x 1 mm; 4 x 0.25 
Super preparative flow cell: 4 mm x 0.15 mm

Maximum flow rate preparative flow cell:

Preparative flow cell: 500 mL/min
Super preparative flow cell: 10 L/min

Pressure:

1,000 psi maximum
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