1260 Infinity Fluorescence Detector
Sensitive and reliable fluorescence detection for HPLC and UHPLC
The proven optical and electronic design of the Agilent 1260 Infinity Fluorescence Detector provides highest sensitivity for the analysis of trace-level components. Time-programmable excitation and emission wavelength switching allows you to optimize the detection sensitivity and selectivity for your specific applications. High-speed detection with up to 74 Hz data rates keeping you pace with the analysis speed of fast LC.
- Lowest limits of detection with a Raman S/N > 3000 (using dark signal noise reference).
- Simplified optical design for optimized baseline stability.
- Up to 100% resolution gain in fast LC using a 75 Hz data acquisition rate.
- Long-life xenon lamp for highest sensitivity. The long-life (> 4000 hours) flash lamp, lamp reference system and efficient light collection ensure constant lamp energy for maximum excitation of fluorophores.
- Easy front access enables fast inspection or exchange of the flow cell.
- Automatic recognition of all flow cell cartridges provides documentation of instrument parameters and helps to comply with GLP.
- Extensive diagnostics, error detection and display with Instant Pilot controller and Agilent Lab Advisor software.
Specifications: infinity Fluorescence Detector 1260 Infinity (G1321C)
Detection type |
Programmable single wavelength (excitation and emission) fluorescence detector |
Performance |
a) Raman (H2O) > 500 (noise reference measured at signal) |
Light source |
Xenon flash lamp, normal mode (20 W), economy mode (5 W), lifetime 4000 hours |
Pulse frequency |
296 Hz for signal mode, 74 Hz for economy mode |
Excitation monochromator |
Concave holographic grating, F/1.6, blaze 300 nm, |
Emission monochromator |
Concave holographic grating, F/1.6, blaze 400 nm, |
Reference system |
Inline excitation measurement |
Time programming |
Response time, PMT gain, baseline behavior (append, free, zero) |
Spectral acquisition |
Excitation or emission spectra, scan speed 28 ms per datapoint, e.g. 0.6 s per spectrum 200-400 nm, 10 nm step |
Step size |
1-20 nm |
Spectra storage |
Limited only by disk space |
Wavelength repeatability |
± 0.2 nm |
Wavelength accuracy |
±3 nm |
Data rate |
74 Hz |
Flow cells |
Standard: 8 µL volume, 20 bar (2 MPa) pressure maximum, quartz. |
Control and data evaluation |
Agilent OpenLAB Chromatography Data System (CDS) ChemStation Edition and EZChrom Edition or Agilent Instant Pilot data evaluation (limited analysis or printing of spectra) |
Environment |
0 – 40 °C constant temperature at <95% humidity (non-condensing) |
Analog outputs |
Recorder/integrator: 100 mV or 1 V, |
Communications |
LAN, Controller-area-network (CAN), RS232, APG remote |
Safety and maintenance |
Extensive diagnostics, error detection and display (through ChemStation and Instant Pilot), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas |
GLP |
Early maintenance feedback (EMF) for continuous tracking of instrument usage, display of feedback messages in terms of lamp burn time with user-settable limits and feedback messages. Electronic records of maintenance and errors. Verification of wavelength accuracy using Raman band (H20) lines. |