Steroids in Plasma / Serum

RECIPE developed a fast and simple quantitative Steroid profiling LC-MS/MS method that covers the most relevant steroid hormones.

Steroid hormones are synthesised from cholesterol. The majority of these powerful molecules are produced by the adrenal glands, testes or ovaries. They fulfil a wide range of physiological functions after binding to specific cell receptors of various target organs. Mainly they are involved in the carbohydrate, lipid and protein metabolism, stress response and in the regulation of the water and mineral balances, in reproductive processes and the development of secondary sex characteristics.

Consequently steroid hormones are also involved in a variety of diseases, like adrenal tumors, the Adrenogenital syndrome (CAH), the Cushing’s syndrome or the Polycystic Ovarian Syndrome. The quantitative profiling of steroids is essential in diagnosis and evaluation of pathologic conditions and therapeutic responses.

Analytical Parameters: 

  • Cortisol
  • DHEAS
  • 21-Deoxycortisol
  • 11-Deoxycortisol
  • Testosteron
  • Androstendion
  • 21-OH Progesteron
  • 17-OH Progesteron

RECIPE developed a fast and simple quantitative Steroid profiling LC-MS/MS method that covers the most relevant steroid hormones.

We have available an user manual for this application. You can request sending an email to clinica@ingenieria-analitica.com 

Order No. Description Quantity
MS12000 ClinMass® Complete Kit for Steroids in Serum / Plasma 1 pce.
  for 200 assays  
  Contents:  
  Autosampler Washing Solution 1 x MS12005
  Mobile Phase A 1 x MS12008
  Mobile Phase B 1 x MS12009
  Internal Standard IS 1 x MS12012
  Serum Calibrator Set, lyophil. (Level 0 – 6) 1 x MS12013
  Sample Preparation Vials 2 x MS12020
  Precipitant P 1 x MS12021
  Manual  
  Separately available components:  
MS12005 Autosampler Washing Solution 1000 ml
MS12008 Mobile Phase A 1000 ml
MS12009 Mobile Phase B 800 ml
MS12012 Internal Standard IS 10 ml
MS12013 Serum Calibrator Set, lyophil. (Level 0 – 6) 7 x 1 x 2 ml
MS12015 Optimisation Mix 1 2 ml
MS12016 Optimisation Mix 2 2 ml
MS12020 Sample Preparation Vials 100 pcs.
MS12021 Precipitant P 5 ml
  Start Accessory:  
MS12030 Analytical Column with test chromatogram 1 pce.
  ClinChek® Controls:  
MS12083 Serum Control, lyophil., 3 x 3 x 2 ml
  Level I, II, III