Quantitative Amino Acids Analysis
Analysis of free amino acids have applications of various fields, at the beginning of this diagnosis of inherited metabolic disorders, feeding newborn with a metabolic disorder inherited comes from studies. Furthermore, amino acid analysis in diagnosis problems such as liver and kidney disorders, intestinal microflora disorder, no problems are performed. 43 amino acids are performed at 7.5 minutes with Jasem quantitative amino acids analysis kit for only one injection. Serum/ plasma and urine samples are becoming ready for analysis at 5 min.
In addition, Jasem method allows diagnosis of maple syrup urine disease (MSUD) by second injection of the same vial from the prepared sample. The second run provides excellent chromatographic separation of allo-isoleucine in order to correct quantification.
Features
-Allows analysis without derivatization
-Fast and simple preparation, no need SPE
-Long lifetime HPLC column
-Shorter analysis time (Analysis time 7.5 min)
-Allow analysis allo-isoleucine for maple syrup disease diagnosis with only second injection (Analysis time 11 min)Maple-syrup urine disease (MSUD) is an inborn error of metabolism characterized by the inherited deficiency of the branched-chain-2-oxoacid dehydrogenase complex which is required in the metabolism of the branched-chain amino acids. The detection of allo-isoleucine allows for positive identification of patients with MSUD reducing the false-positive neonatal screening results. Chromatographic separation of allo-isoleucine is readily achieved in 11 minutes analysis time with the accurate quantification using same sample.
Parameters
QUANTITATIVE AMINO ACIDS ANALYSIS: Alanine, arginine, aspartic acid, asparagine, 2-amino-butyric acid, argininosuccinic acid, anserine, 2-amino-adipic acid, 3-amino-isobutyric acid, beta-alanine, ethanolamine, phenylalanine, phosphoserine, o-phosphroyl ethanolamine, gamma aminobutyric acid, glutamic acid, glycine, glutamine, hydroxyproline, homocitrulline, homocysteine, histidine, hyroxylysine, isoleucine, carnosine, lysine, leucine, methionine, 3-methylhistidine, 1-methylhistidine, norvaline, ornithine, proline, cystine, cystathionine, sarcosine, citrulline, serine, taurine, tryptophan, tyrosine, threonine, valine
Matrix: Serum-Plasma-Urine
Sample Preparation for Serum, Plasma and Urine
- Pipet 50 μl serum or urine sample into a centrifuge tube.
- Add 50 μl internal standard solution and 700 μl Reagent-1 onto sample and vortex for 10 seconds.
- Centrifuge at 4000 rpm for 5 minutes.
- Decant the supernatant into HPLC vial and inject into the system.
