Analysis of Urinary Organic Acids
Human urine is a complex biofluid of diverse small polar metabolites such as organic acids. Organic acidurias are inborn errors of metabolism given rise to by defections of inherited enzymes which lead to accumulation excess amounts of normal organic acids in human urine. For this reason, qualitative and quantitative analyses of urinary organic acids are prominent diagnostic tools for the interpretation of metabolite profile that reflects inherited metabolic disorders.
Features
- Analysis of 54 organic acids without derivatization.
- Quick and easy sample preparation; just dilution!
- 12 minutes run for each panel (Total analysis time 24 minutes)
- Successful chromatographic separation of isomers.
Matrix: Urine
Conventionally gas chromatography coupled to mass spectrometry (GC-MS) is used despite disadvantages as limitation to volatile and thermally stable analytes thereby obligating of time-consuming derivatization process.
At this point, “Jasem - Organic Acids” method overcomes above mentioned drawbacks by using liquid chromatography tandem mass spectrometry (LC-MS/MS). This method ensures quantification of 54 organic acids by absence of derivatization simplified sample preparation to diluting. Also, offers two panels achieving sufficient chromatographic separation for crucial isomers by LC-MS/MS.
Sample Preparation for Urine
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Dilute urine sample 10 times using Reagent-1. -
Transfer 100 µL of diluted urine sample to a vial. -
Add 50 µL of IS mix and 850 µL of Reagent-1 into the sample and inject to LC-MS/MS system.
