25-OH Vitamin D2 / D3 in Plasma / Serum
The ClinMass® Complete Kits with order nos. MS7000, MS7100 and MS7200 enable a fast on-line SPE LC-MS/MS analysis of 25-OH-Vitamin D (D2, D3) with an injection interval of 3 min only.
On-line Analysis
The C-3 epimer of 25-OH vitamin D3 (3-epi-25-OH vitamin D3) is separated reliably from the analyte peak. An analytical column with a run time of 4 min (order no. MS7035) is additionally available in order to obtain baseline separation.
Automated on-line Analysis
In order to meet laboratory demands in high sample throughput for Vitamin D RECIPE has developed a fully automated sample preparation kit for many liquid handling devices with the option to use 96-well-filter-plates as well. The C-3 epimer of 25-OH vitamin D3 (3-epi-25-OH vitamin D3) is separated reliably from the analyte peak. An analytical column with a run time of 4 min (order no. MS7035) is additionally available in order to obtain baseline separation.
We have available an user manual for this application. You can request sending an email to clinica@ingenieria-analitica.com
Order No. |
Description |
Quantity |
MS7100 |
ClinMass® Complete Kit for 25-OH-Vitamin D2/D3 in Plasma and Serum for 1200 assays |
1 pce. |
Kit Components (order no. MS7100): |
||
MS7005 |
Autosampler Washing Solution |
4 x MS7005 (4 x 1000 ml) |
MS7009 |
SPE-Buffer |
8 x MS7009 (8 x 1000 ml) |
MS7010 |
Mobile Phase |
4 x MS7010 (4 x 700 ml) |
MS7012 |
IS Internal Standard |
4 x MS7012 (4 x 30 ml) |
MS7013 |
Serum Calibrator Set, lyophil. (Level 0 – 3) |
4 x MS7013 (MS7013: 4 x 1 x 1 ml) |
FK6513 |
96-Well-Plates (600 µl) |
3 x FK6513 (3 x 5 pcs.) |
MS7021 |
P Precipitant |
4 x MS7021 (4 x15 ml) |
|
Manual |
1 pce. |
Additional Components: |
||
MS7014 |
Optimisation Mix |
2 ml |
MS7015 |
Optimisation Mix 3-epi |
1 ml |
Start Accessories: |
||
MS7030 |
Analytical Column with test chromatogram |
1 pce. |
MS7035 |
Analytical Column 3-epi with test chromatogram |
1 pce. |
|
(for the additional analysis of 3-epi-25-OH-Vitamin D3) |
|
MS7031 |
SPE-Column |
1 pce. |
Accessories: |
||
FK6501 |
Protective sheets for 96-well-plate (PE/PP foil, 80 x 140 mm) |
50 pcs. |
FK6514 |
96-Well-Plates (300 µl) |
5 pcs. |
FK7340 |
Sealings and sieves |
4 pcs. each |
FK7400 |
Inline-Filter (stainless steel sieve, free of dead volume) |
1 pce. |
ClinChek® Controls: |
||
MS7080 |
Serum Control, lyophil., Level I |
10 x 1 ml |
MS7081 |
Serum Control, lyophil., Level II |
10 x 1 ml |
MS7082 |
Serum Control, lyophil., Level I, II |
2 x 5 x 1 ml |