25-OH Vitamin D2 / D3 in Plasma / Serum

The ClinMass® Complete Kits with order nos. MS7000, MS7100 and MS7200 enable a fast on-line SPE LC-MS/MS analysis of 25-OH-Vitamin D (D2, D3) with an injection interval of 3 min only.

On-line Analysis

The C-3 epimer of 25-OH vitamin D3 (3-epi-25-OH vitamin D3) is separated reliably from the analyte peak. An analytical column with a run time of 4 min (order no. MS7035) is additionally available in order to obtain baseline separation.

Automated on-line Analysis

In order to meet laboratory demands in high sample throughput for Vitamin D RECIPE has developed a fully automated sample preparation kit for many liquid handling devices with the option to use 96-well-filter-plates as well. The C-3 epimer of 25-OH vitamin D3 (3-epi-25-OH vitamin D3) is separated reliably from the analyte peak. An analytical column with a run time of 4 min (order no. MS7035) is additionally available in order to obtain baseline separation.

We have available an user manual for this application. You can request sending an email to clinica@ingenieria-analitica.com

Order No.

Description

Quantity

MS7100

ClinMass® Complete Kit for 25-OH-Vitamin D2/D3 in Plasma and Serum for 1200 assays

1 pce.

Kit Components (order no. MS7100):

MS7005

Autosampler Washing Solution

4 x MS7005 (4 x 1000 ml)

MS7009

SPE-Buffer

8 x MS7009 (8 x 1000 ml)

MS7010

Mobile Phase

4 x MS7010 (4 x 700 ml)

MS7012

IS Internal Standard

4 x MS7012 (4 x 30 ml)

MS7013

Serum Calibrator Set, lyophil. (Level 0 – 3)

4 x MS7013 (MS7013: 4 x 1 x 1 ml)

FK6513

96-Well-Plates (600 µl)

3 x FK6513 (3 x 5 pcs.)

MS7021

P Precipitant

4 x MS7021 (4 x15 ml)

 

Manual

1 pce.

Additional Components:

MS7014

Optimisation Mix

2 ml

MS7015

Optimisation Mix 3-epi

1 ml

Start Accessories:

MS7030

Analytical Column with test chromatogram

1 pce.

MS7035

Analytical Column 3-epi with test chromatogram

1 pce.

 

(for the additional analysis of 3-epi-25-OH-Vitamin D3)

 

MS7031

SPE-Column

1 pce.

Accessories:

FK6501

Protective sheets for 96-well-plate (PE/PP foil, 80 x 140 mm)

50 pcs.

FK6514

96-Well-Plates (300 µl)

5 pcs.

FK7340

Sealings and sieves

4 pcs. each

FK7400

Inline-Filter (stainless steel sieve, free of dead volume)

1 pce.

ClinChek® Controls: 

MS7080

Serum Control, lyophil., Level I

10 x 1 ml

MS7081

Serum Control, lyophil., Level II

10 x 1 ml

MS7082

Serum Control, lyophil., Level I, II

2 x 5 x 1 ml