Products

1260 Infinity Bio-inert High-Performance Autosampler

The Agilent 1260 Infinity Bio-inert High-performance Autosampler is ideal for all bio-related applications, such as protein or oligonucleotide analysis. It features injector programming for online dilution and sample pretreatment, for example, for fluorescence labeling. The ceramic needle, PEEK needle seat and wash, and stainless steel- clad PEEK capillaries ensure highest injection accuracy and precision with minimum carryover. Based on the Agilent 1260 Infinity High Performance Autosampler, specifically designed for resolution, speed, sensitivity, and low carryover it offers maximum flexibility and fast injection cycles. To protect labile compounds from degradation during analysis or storage it can be upgraded to a thermostatted version by adding the Agilent 1290 Infinity Thermostat.Together with the Agilent Bio-HPLC column portfolio for SEC and IEX, highest resolution per time is achieved for protein and NBE characterization.

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  • Metal free sample flow path at 600 bar, no precious sample touches metal surfaces

  • Novel capillary and connection design

  • Low carryover of 40 ppm

  • Handles vials and microtiter plates and injects volumes from 0.1 – 100 μL,extendable to 1.5 mL.

  • Injector programming for online dilution and sample pretreatment, eg for fluorescence labeling

  • Peltier temperature control from 4 to 40° C for thermally labile samples (upgrade from non-thermostted to thermostatted version possible).

  • Superior injection precision up to 100 µL, higher injection volumes possible with multi-draw kit

  • Controlled by OpenLab CDS Software

 Agilent 1260 Infinity Bio-inert High-Performance Autosampler (G5667A)

Type

Specifications

GLP features

Early maintenance feedback (EMF), electronic records of maintenance and errors

Communications

Controller-area network (CAN). RS232C, APG-remote standard, optional four external contact closures and BCD vial number output

Safety features

Leak detection and safe leak handling, low voltages in maintenance areas, error detection and display

Injection range

0.1 – 100 μL in 0.1 μL increments. Up to 40 μL with reduced injection volume kit (hardware modification required). Up to 1500 μL with multiple draw (hardware modification required)

Precision

Typically < 0.25% RSD from 5 – 100 μL
Typically < 0.5% RSD from 2 – 5 μL
Typically < 0.7% RSD from 1 – 2 μL volume
Measured with injections of benzylalcohol

Pressure range

Up to 600 bar (8700 psi)

Sample viscosity range

0.2 – 5 cp

Sample capacity

2 × well plates (MTP) + 10 × 2 mL vials 108 x 2-mL vials in2 x 54 vial plate plus 10 additional 2 mL vials
30 x 6-mL vials in 2 x 15 vial plate plus 10 additional 2 mL vials
54 Eppendorf tubes (0.5/1.5/2.0mL) in 2 x 27 Eppendorf tube plates

Injection cycle time

Typically < 17 s using the following standard conditions:
Default draw speed: 100 μL/min 
Default eject speed: 100 μL/min 
Injection volume: 5 μL

Carry-over

Typically < 0.004% using the following conditions:
Column: Agilent ZORBAX SB-C18, 21 x 50 mm 1.8 μm
Mobile Phase: A: H2O + 0.05 % TFA, B: ACN+ 0.045 TFA
Gradient: 0.1 min 10% B, 3.1 min 90% B, 3.2 min 90 % B, 3.21 10 %B, 4.5min stop
Flow rate: 0.5 mL/min
Temperature: 25 °C
Wavelength: 257 nm
Sample : 1200 ng/µL Chlorhexidine (disolved in H2O with 0.1% TFA), 1 µL injected and measured both on Agilent 6410 QQQ and Agilent DAD (G1315C)
Wash solution: H2O with 0.1% TFA (5 sec)

pH-range

1-13 (short term 14)

Materials used in sample flow path

Needle seat

PEEK

Needle

Ceramic

Sample loop

Metal-cladded (outside) PEEK (inside)

Injection valve

PEEK, ceramic, FFKM

Other

PPSO2, PTFE

Sample cooling

Optional with G1330B 4 °C – 40 °C
Portable Electronic Nose PEN3

Portable Electronic Nose PEN3