- Descripción detallada
This preliminary study evaluates the use of GC×GC with parallel detection by flame ionisation detection (FID) and TOF MS for an extended list of 84 allergens. This enables robust quantitation and confident identification in a single run, making it an ideal system for R&D labs requiring full sample characterisation. Moreover, once method optimisation and validation is complete, the excellent repeatability of flow modulation allows the method to be translated easily across GC×GC–FID systems in multiple quality-control laboratories.
Sample preparation: A series of calibration standards ranging from 3.2–400 μg/mL were prepared for a mixture of 84 allergens (plus 1,4 - dibromobenzene as an internal standard) in methyl tert-butyl ether (MTBE). The essential oils and the perfume mix diluted in MTBE to 0.5% and 1.5%, respectively.
GC×GC: Injector- Split/splitless; Injection volume- 1.0 μL; Split 25:1. Flow modulator- INSIGHT (SepSolve Analytical). A splitter used to direct the flow to the TOF MS and FID detectors in the ratio 1:4.
TOF MS: Instrument BenchTOF-Select (Markes International).
Software: ChromSpace GC×GC software (Markes International).