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220-001
Attomole detection limits in micro HPLC-ECD
Introduction
Since the introduction in the early 70'ies, electrochemical detection (ECD) has developed to a reliable, sensitive and selective detection method for HPLC. The technique has found its way in many laboratories in fields of neurochemistry, pharmaceutical chemistry, food chemistry and environmental chemistry. The versatility of the technique is illustrated by many applications [1-5]. Characteristic for ECD are the low limits of detection that can be obtained, especially in combination with micro HPLC. Unlike most other detection techniques, detection limits in ECD do not deteriorate in miniaturised systems, they in fact may improve considerably [6]. This not only holds for mass detection limits, which improve (with limited practical use) because of the smaller injection volume, but also for concentration detection limits. In this Technical Note peak heights and signal-to-noise ratios in microbore HPLC are compared to standard HPLC with ECD. Improvements in concentration detection limits by a factor 6-10 are demonstrated. Under the current conditions the minimum detectable amount for dopamine is 160 attomole (25 fg).
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